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Miami Beach Sources of EnterococciSources of Enterococci to Park View Canal in Miami Beach Presentation given by Helena Solo-Gabriele, Ph.D., P.E. Professor, University of Miami March 27, 2023 Larissa Montas, Ph.D. Afeefa Abdool-Ghany, M.S. Yutao Chen, M.S. Erik Lamm Ashley Quijada Rivka Reiner Hekai Zhang, M.S. N Park View Canal (PVC) is a secondary canal with limited flow located within Biscayne Bay. Biscayne Bay Historic Enterococci Concentrations in PVC Canal 70 Objectives ●From where are the enterococci coming from? ●How enterococci from humans, dogs, and birds enter the waterway? ○Geographic area ○Fresh versus marine water ○Environmental conditions associated with elevated enterococci (rain, tide, sunlight) Our Approach Sampling Efforts Included: •Evaluate Historical Records (Miami Beach has made considerable efforts) •Initiate Sampling Program ●Intense Spatial Sampling at the PVC ●Storm Water System ●Shoreline Sediments ●Intense Temporal Sampling ●Depth Sampling Historical records show: enterococci associated with •Rainy days •Fresher waters Sampling Effort ●Intense Spatial Sampling at the PVC ●Storm Water System ●Shoreline Sediments ●Intense Temporal Sampling ●Depth Sampling Spatially Intense Sampling High Tide, August 9 (dry) (Saltier Water) Kayak Launch Hot Spot Storm Outfalls Low Tide, September 16 (wet) (Fresher Water) Sampling Effort ●Intense Spatial Sampling at the PVC ●Storm Water System ●Shoreline Sediments ●Intense Temporal Sampling ●Depth Sampling Intense Storm Water Conveyance and Sanitary Infrastructure Initial Sites Background Sites Exploratory Sites Kayak Launch 1 2 3 456 8 9 7 10 12 13 14 1516 1 7 10 ~100 CFU/100 mL ~100 CFU/100 mL T1 B1 T2 B2 T3 T6 B6 T5 B5 T4 T1,T2…Top sediments B1,B2…Bottom sediments S1,S2,S3 Waterway bank S1 S2 S3 What Are Possible Contribution From Sedime Storm Water Inlet Top Sediments Bottom Sediments Shoreline Sediments > 360,000 CFU/g > 650,000 CFU/g > 790,000 CFU/g Sampling Efforts ●Intense Spatial Sampling at the PVC ●Storm Water System ●Shoreline Sediments ●Intense Temporal Sampling ●Depth Sampling Time Intense Sampling Started to Rain Above >24,000 Depth Intense Sampling Measurements in the vertical direction within the water column at: 0, 1, 2, 3, 4, and 5 feet.Highest Level at Surface (Freshwater) Sanitary Sewer ? (fresh water) Not proven but possible Main Source is Fresh Water Carried by the Storm Water Conveyance System Storm Water Inlet (fresh water) Salty Saltier water (lower bacteria) Fresher water (high bacteria) Long term impactful solutions: Upgrade storm water conveyance system (treat first flu Upgrade sanitary sewer system (continuous checking/mainte HomelessMore Frequent Cleaning Garbage Bins Leaking Uncovered Animal Feces Dogs Iguanas Racoons Birds Short term items Conclusions/Summary Enterococci is: ●Associated with freshwater and this freshwater floats on canal surface ●Enters during storm events through stormwater conveyance system ●Source to stormwater includes: ○Animal feces (domestic and feral) ○Homeless without access to sanitation ○Leaking garbage bins ○Trash ●Cannot discount sanitary sewage. When sewage backs up and overflows it enters the stormwater conveyance system and contaminates the streets including all sediments ●Need to further investigate underground sewage leaks. Such leaks can possibly impact the stormwater conveyance system. Key Take-aways ●Short term. Housekeeping on streets (initiated by City during course of study) ●Long term. Investments needed in upgrading ○the stormwater conveyance system and ○the sanitary sewer system . Thank you. hmsolo@miami.edu